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gel doc 2000  (Bio-Rad)


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    Structured Review

    Bio-Rad gel doc 2000
    Gel Doc 2000, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 2482 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gel+doc+2000/Filter/pmc11880981-238-30-33
    Average 96 stars, based on 2482 article reviews
    gel doc 2000 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    other:

    Article Title: Investigation of genetic diversity using molecular and biochemical markers associated with powdery mildew resistance in different flax ( Linum usitatissimum L.) genotypes
    Article Snippet: The gel was photographed, scanned, and analyzed using the Gel Doc 2000 Bio-Rad apparatus.

    Polymerase Chain Reaction:

    Article Title: Peptidylarginine deiminase 3 modulates response to neratinib in HER2 positive breast cancer
    Article Snippet: .. After PCR, the amplified fragments were separated on agarose gels and the bands were visualized in a Gel Doc 2000 (Bio-Rad Laboratories, Hercules, CA, USA) apparatus. .. Bands were excised and amplified DNAs purified using the GFXTM PCR DNA and gel Band purification KIT (GE Healthcare, Buckinghamshire, United Kingdom).

    Article Title: Comparison of a multiplex PCR with DNA barcoding for identification of container breeding mosquito species
    Article Snippet: PCR reactions consisted of 10 μL REDTaq Ready Mix (Merck, Darmstadt, Germany), 7 μL nuclease-free water, 0.4 μL of each primer (10 μM) and 1 μL of DNA, while the PCR conditions described by Bang et al. [ ] were applied (94 °C/5 min; then 94 °C/30 s, 56 °C/30 s, 72 °C/30 s for 35 cycles; and the final extension at 72 °C for 5 min). .. Gel electrophoresis (gel: 2.0% agarose gel in 0.5× TBE buffer; voltage: 100 V; run time: 90–120 min) and visualisation with the Gel Doc 2000 (Bio-Rad Laboratories, Hercules, USA) was performed to identify potential PCR products. .. The GeneRuler 100 bp DNA Ladder (Thermo Fisher Scientific, Waltham, USA) was used for sizing of PCR products.

    Article Title: Sustained aviremia despite anti-retroviral therapy non-adherence in male children after in utero HIV transmission.
    Article Snippet: After sporadic reports of post-treatment control of HIV in children who initiated combination anti-retroviral therapy (cART) early, we prospectively studied 284 very-early-cART-treated children from KwaZulu-Natal, South Africa, after vertical HIV transmission to assess control of viremia.. Eighty-four percent of the children achieved aviremia on cART, but aviremia persisting to 36 or more months was observed in only 32%.. We observed that male infants have lower baseline plasma viral loads (P = 0.01).

    Amplification:

    Article Title: Peptidylarginine deiminase 3 modulates response to neratinib in HER2 positive breast cancer
    Article Snippet: .. After PCR, the amplified fragments were separated on agarose gels and the bands were visualized in a Gel Doc 2000 (Bio-Rad Laboratories, Hercules, CA, USA) apparatus. .. Bands were excised and amplified DNAs purified using the GFXTM PCR DNA and gel Band purification KIT (GE Healthcare, Buckinghamshire, United Kingdom).

    Nucleic Acid Electrophoresis:

    Article Title: Comparison of a multiplex PCR with DNA barcoding for identification of container breeding mosquito species
    Article Snippet: PCR reactions consisted of 10 μL REDTaq Ready Mix (Merck, Darmstadt, Germany), 7 μL nuclease-free water, 0.4 μL of each primer (10 μM) and 1 μL of DNA, while the PCR conditions described by Bang et al. [ ] were applied (94 °C/5 min; then 94 °C/30 s, 56 °C/30 s, 72 °C/30 s for 35 cycles; and the final extension at 72 °C for 5 min). .. Gel electrophoresis (gel: 2.0% agarose gel in 0.5× TBE buffer; voltage: 100 V; run time: 90–120 min) and visualisation with the Gel Doc 2000 (Bio-Rad Laboratories, Hercules, USA) was performed to identify potential PCR products. .. The GeneRuler 100 bp DNA Ladder (Thermo Fisher Scientific, Waltham, USA) was used for sizing of PCR products.

    Agarose Gel Electrophoresis:

    Article Title: Comparison of a multiplex PCR with DNA barcoding for identification of container breeding mosquito species
    Article Snippet: PCR reactions consisted of 10 μL REDTaq Ready Mix (Merck, Darmstadt, Germany), 7 μL nuclease-free water, 0.4 μL of each primer (10 μM) and 1 μL of DNA, while the PCR conditions described by Bang et al. [ ] were applied (94 °C/5 min; then 94 °C/30 s, 56 °C/30 s, 72 °C/30 s for 35 cycles; and the final extension at 72 °C for 5 min). .. Gel electrophoresis (gel: 2.0% agarose gel in 0.5× TBE buffer; voltage: 100 V; run time: 90–120 min) and visualisation with the Gel Doc 2000 (Bio-Rad Laboratories, Hercules, USA) was performed to identify potential PCR products. .. The GeneRuler 100 bp DNA Ladder (Thermo Fisher Scientific, Waltham, USA) was used for sizing of PCR products.

    Article Title: Sustained aviremia despite anti-retroviral therapy non-adherence in male children after in utero HIV transmission.
    Article Snippet: After sporadic reports of post-treatment control of HIV in children who initiated combination anti-retroviral therapy (cART) early, we prospectively studied 284 very-early-cART-treated children from KwaZulu-Natal, South Africa, after vertical HIV transmission to assess control of viremia.. Eighty-four percent of the children achieved aviremia on cART, but aviremia persisting to 36 or more months was observed in only 32%.. We observed that male infants have lower baseline plasma viral loads (P = 0.01).

    Electrophoresis:

    Article Title: Molecular Detection of Macrolide-Induced Clindamycin Resistance Among Clinical Isolates of Staphylococcus aureus from Selected Hospitals in Katsina Metropolis
    Article Snippet: .. Following electrophoresis, the gel was visualized using a gel documentation system, specifically the BIO-RAD Gel Doc 2000, allowing for the visualization and documentation of the DNA bands. ..

    Membrane:

    Article Title: Expression and potential molecular mechanism of TOP2A in metastasis of non-small cell lung cancer.
    Article Snippet: TOP2A and Wnt3a antibodies were manufactured by Abcam, and additional antibodies were manufactured by Boster. .. The following day, the membrane was washed three times with TBST for 8 min on a shaker, incubated for 1.5 h with secondary antibodies, and photographed using Gel Doc 2000 (Bio-RAD) equipment. ..

    Incubation:

    Article Title: Expression and potential molecular mechanism of TOP2A in metastasis of non-small cell lung cancer.
    Article Snippet: TOP2A and Wnt3a antibodies were manufactured by Abcam, and additional antibodies were manufactured by Boster. .. The following day, the membrane was washed three times with TBST for 8 min on a shaker, incubated for 1.5 h with secondary antibodies, and photographed using Gel Doc 2000 (Bio-RAD) equipment. ..



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